 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Morphological Features of Porcine Oviductal Epithelial Cells and Cumulus-Oocyte Complex) ผู้เขียน: Mayuva Areekijseree,  นางอมรา ทองปาน, รองศาสตราจารย์ , นางเรณู เวชรัชต์พิมล สื่อสิ่งพิมพ์:pdf AbstractPorcine oviductal epithelial cells (POEC) and cumulus-oocyte complexes (COCs) were observed using inverted microscopy and scanning electron microscopy. At follicular phase, POEC contained a great number of long ciliated cells whereas those at luteal phase consisted mostly of round shaped nonciliated cells with short microvilli on the apical surface. This change in morphological features of POEC seemed to serve well on their functions as oocyte transporters at follicular phase. As for COCs, they were morphologically classified into 4 types based on the accumulation and arrangement of cumulus cells around the oocytes. These were intact cumulus cell layer, single cumulus cell layer, partial cumulus cell layer and completely denuded oocytes at the percentages of composition of 19.87%, 18.13 %, 28.88 % and 33.12 %, respectively. Cumulus cells attached on the surface of oocytes were teardrop-like shape having the conical ends pointed towards the oocytes membrane surface while free-floating cumulus cells in the follicular fluid and those at the outer layers were round in shape. These POEC and COCs were high potential feeder cells and could be further cultured for in vitro fertilization use. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Activity Profiles at Different pH and Temperature of Cellulases and Lipases in Freshwater Pearl Mussel: Hyriopsis (Hyriopsis) bialatus, Simpson 1900) ผู้เขียน:Mayuva Areekijseree, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, ดร.อุทัยวรรณ โกวิทวที, รองศาสตราจารย์ , นางอมรา ทองปาน, รองศาสตราจารย์, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ , สาธิต โกวิทวที สื่อสิ่งพิมพ์:pdf AbstractThe enzymatic properties of two digestive enzymes, cellulases and lipases, from stomach and intestine of adult freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus, Simpson 1900, were studied at various pH (1.0-11.0) and temperatures (20-80?C). Activity profiles of both enzymes in male and female mussels at various pH and temperature were similar. Optimum pH of cellulases in stomach and intestine was found to be 6.0 while optimum temperature was 35-55?C in stomach and 30-60?C in intestine. Lipase activity was low in intestine but its optimum pH in both organs was found at pH 8.0 and optimum temperature at 35-55?C. Both cellulase and lipase showed higher activities in the stomach than in intestine. At habitat temperature (28-30?C) of Thai freshwater pearl mussel species, cellulase and lipase specific activities dominated in stomach. |